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"First class oysters" : progress and constgraints in genetic improvement of the Pacific oyster (Crassostrea gigas) ArchiMer
Boudry, Pierre.
The most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) up until now has been obtained through the production of triploids, particularly since the development of tetraploids in the mid 90s. Alternatively, quantitative genetics studies suggest that significant gains could be obtained in traits of aquacultural interest. However, the limited extent of hatchery propagation (compared with natural recruitment) in some countries and/or technical difficulties and biological characteristics of this species have retarded the development of selective breeding programs for C. gigas. Individual selection can however be performed easily in such a highly fecund species, though it often leads to small effective population sizes...
Tipo: Text Palavras-chave: Selective breeding; Genetic; Crassostrea gigas; Pacific oysters.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3462.pdf
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Fitness landscapes support the dominance theory of post-zygotic isolation in the mussels Mytilus edulis and M. galloprovincialis ArchiMer
Bierne, Nicolas; Bonhomme, Francois; Boudry, Pierre; Szulkin, M; David, Pierre.
We studied the genetic basis of post-zygotic isolation in the marine mussels Mytilus edulis and Mytilus galloprovincialis. Evidence was obtained for a high number of recessive Dobzhansky-Muller substitutions in the genome of these two mussel taxa. We analysed the segregation of unlinked diagnostic markers in the progeny of two backcrosses and an F-2 cross, 36 h and 200 days after fertilization. Directional selection favouring M. galloprovincialis genotypes was observed in both kinds of cross. In the F-2, epistatic interactions between each pair of chromosome fragments mapped by the markers were identified in addition. Our results imply that homozygous-homozygous interactions are required for breakdown of coadaptation, in accordance with the dominance...
Tipo: Text Palavras-chave: Mytilus; Dobzhansky Muller incopatibilities; Post zygotic isolation; Negative epistasis; Hybrid breakdown; Hybrid zone.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1350.pdf
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Forty questions of importance to the policy and practice of native oyster reef restoration in Europe ArchiMer
Zu Ermgassen, Philine S. E.; Bonačić, Kruno; Boudry, Pierre; Bromley, Cass A.; Cameron, Tom C.; Colsoul, Bérenger; Coolen, Joop W. P.; Frankić, Anamarija; Hancock, Boze; Have, Tom M.; Holbrook, Zoë; Kamermans, Pauline; Laugen, Ane T.; Nevejan, Nancy; Pogoda, Bernadette; Pouvreau, Stephane; Preston, Joanne; Ranger, Christopher J.; Sanderson, William G.; Sas, Hein; Strand, Åsa; Sutherland, William J..
Oyster reefs are among the most threatened marine habitats globally. In Europe, oyster reefs have been extirpated from most locations within their historical range. Active restoration of the native oyster (Ostrea edulis) in Europe has grown substantially in recent years. In sharing experiences between oyster restoration projects in Europe at the Native Oyster Restoration Alliance conference, NORA2, in Edinburgh in May 2019, it became apparent that a number of similar barriers are experienced. This study identified the top 40 questions, which, if answered, would have the greatest influence on the policy and practice of oyster restoration in Europe. Initially 71 people were consulted across 28 institutions and 11 European countries to generate 194 questions....
Tipo: Text Palavras-chave: Alien species; Estuary; Fishing; Invertebrates; Pollution; Restoration; Subtidal.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00658/77004/78261.pdf
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From Crassostrea gigas oyster larvae adhesion studies to potential biotechnological development of marine adhesives ArchiMer
Foulon, Valentin; Boudry, Pierre; Guerard, Fabienne; Hellio, Claire.
Tipo: Text
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00373/48449/48717.pdf
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Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas ArchiMer
Bigot, Laetitia; Beets, Isabel; Dubos, Marie-pierre; Boudry, Pierre; Schoofs, Liliane; Favrel, Pascal.
Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the identification of a receptor (CgsNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC50 values...
Tipo: Text Palavras-chave: Oyster; Crassostrea gigas; SNPF; Energy metabolism; Reproduction; Starvation.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00207/31777/30188.pdf
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Gametogenesis in the Pacific Oyster Crassostrea gigas: A Microarrays-Based Analysis Identifies Sex and Stage Specific Genes ArchiMer
Dheilly, Nolwenn; Lelong, Christophe; Huvet, Arnaud; Kellner, Kristell; Dubos, Marie-pierre; Riviere, Guillaume; Boudry, Pierre; Favrel, Pascal.
Background: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and little more about the molecular pathways regulating reproduction. We have recently developed and validated a microarray containing 31,918 oligomers (Dheilly et al., 2011) representing the oyster transcriptome. The application of this microarray to the study of mollusk gametogenesis should provide a better understanding of the key factors involved in sex differentiation and the regulation of oyster reproduction. Methodology/Principal Findings: Gene expression was...
Tipo: Text
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00087/19862/17505.pdf
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Generation and analysis of a 29,745 unique Expressed Sequence Tags from the Pacific oyster (Crassostrea gigas) assembled into a publicly accessible database: the GigasDatabase ArchiMer
Fleury, Elodie; Huvet, Arnaud; Lelong, Christophe; De Lorgeril, Julien; Boulo, Viviane; Gueguen, Yannick; Bachere, Evelyne; Tanguy, Arnaud; Moraga, Dario; Fabioux, Caroline; Lindeque, Penelope; Shaw, Jenny; Reinhardt, Richard; Prunet, Patrick; Davey, Grace; Lapegue, Sylvie; Sauvage, Christopher; Corporeau, Charlotte; Moal, Jeanne; Gavory, Frederick; Wincker, Patrick; Moreews, Francois; Klopp, Christophe; Mathieu, Michel; Boudry, Pierre; Favrel, Pascal.
Background: Although bivalves are among the most-studied marine organisms because of their ecological role and economic importance, very little information is available on the genome sequences of oyster species. This report documents three large-scale cDNA sequencing projects for the Pacific oyster Crassostrea gigas initiated to provide a large number of expressed sequence tags that were subsequently compiled in a publicly accessible database. This resource allowed for the identification of a large number of transcripts and provides valuable information for ongoing investigations of tissue-specific and stimulus-dependant gene expression patterns. These data are crucial for constructing comprehensive DNA microarrays, identifying single nucleotide...
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6770.pdf
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Genetic and reproduction strategy in Crassostrea gigas mortality ArchiMer
Moal, Jeanne; Bedier, Edouard; Fleury, Pierre-gildas; Langlade, Aime; Le Coguic, Yvette; Degremont, Lionel; Boudry, Pierre; Le Coz, Jean-rene; Pouvreau, Stephane; Enriquez-diaz, M.; Lambert, Christophe; Quere, Christele; Soudant, Philippe; Samain, Jean-francois.
Bi-parental families were produced in hatchery and tested in the field in 2001. Two sets of 5 families were constituted, selected on their high (R) and low (S) survival. These two sets were reared in Brittany from March to November 2002. Samplings were performed twice a month to obtain data on biometry, survival, reproductive cycle, biochemical composition, adenylate energy charge, hemolymph parameters (ions and defense system) and muscle strength. "R" and "S" oysters exhibited different reproductive effort and spawning strategy. "R" oysters allocated less energy in gonad than "S" ones and presented a complete spawning at the end of August contrary to the "S" which spawns partially. Mortality started in July when the seawater temperature reached 19 degree...
Tipo: Text Palavras-chave: Summer; Reproduction; Oyster culture; Mortality; Genetic diversity; Commercial species; Breeding.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3345.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - First Progress Report 1st January-31st December 1996 - Contract n° FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Hawkins, A. J. S.; Thiriot Quievreux, Catherine; Wilkinns, N. P.; Zouros, Eleftherios; Bonhomme, Francois.
The EEC project "Genephys 1996-2000", initiated by the IFREMER laboratory GAP (Génetique, Aquaculture et Pathologie, La Tremblade and CREMA (Centre de Recherche en Ecologie Marine et Aquaculture, l'Houmeau), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main objectives are: 1- To express the maximum variability of growth, both at the larval stage and at the adult stage, in an oyster population originating from parents of various origins. 2- To check the persistence over the time, of growth performance and related physiological functions. 3-...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Absorption efficiency; Growth; Oysters; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00050/16149/13632.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - Second Progress Report 1st January-31st December 1997 - Contract n° FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Hawkins, A. J. S.; Thiriot Quievreux, Catherine; Wilkins, N. P.; Zouros, Eleftherios; Bonhomme, Francois; Buestel, Dominique; Samain, Jean-francois.
The UE project "GENEPHYS", initiated by the IFREMER laboratory GAP-La Tremblade (Génétique Aquaculture et Pathologie) and CREMA-L'Houmeau (Centre de Recherche en Ecologie marine et Aquaculture), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main objectives are : 1. To express the maximum variability of growth, both at the larval stage and at the adult stage, in a oyster population originating from parents of various origins. 2. To check the persistence over the time, of growth performance and related physiological functions. 3. To explain...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Absorption efficiency; Growth; Oysters; Crassostrea gigas.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00050/16150/13633.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - Third Progress Report 1st January-31st December 1998 - Contract N°FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Buestel, Dominique; Samain, Jean-francois; Hawkins, A. J. S.; Wilins, N.; Zouros, Eleftherios; Bonhomme, Francois; Thiriot Quievreux, Catherine.
The EU project "GENEPHYS", initiated by the IFREMER laboratory GAP-La Tremblade (Génétique Aquaculture et Pathologie) and CREMA-l'Houmeau (Centre de Recherche en Ecologie Marine et Aquaculture), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main bjectives are : 1. To express the maximum variability of growth, both at the larval stage and at the adult stage, in a oyster population originating from parents of various origins. 2. To check the persistence over the time, of growth performance and related physiological functions. 3. To explain...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Growth; Absorption efficiency; Oysters; Crassostrea gigas.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00050/16151/13634.pdf
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Genetic basis of gametogenesis in diploid and triploid Pacific oysters, Crassostrea gigas ArchiMer
Normand, Julien; Ernande, Bruno; Boudry, Pierre.
Today, triploidy is the most common method used to genetically improve marine bivalve aquaculture production. In the Pacific oyster Crassostrea gigas, triploidy affects many traits. The most proeminent one is reproductive effort, which is greatly reduced compared to diploids. The combined effects of physiological trade-offs between survival, growth and reproduction together with the reduction of reproductive effort in triploids explains the global enhancement of yield observed. However, the sterility of triploid oysters is only partial and some triploid individuals can show normal gametogenesis (i.e. similar to diploids), especially under warm conditions. In diploid oysters, the heritability of reproductive effort has been shown to be relatively low, but a...
Tipo: Text Palavras-chave: Parentage analysis; Triploidy; Oyster; Quantitative genetics; Gametogenesis.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-4525.pdf
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Genetic basis of growth, survival and their plasticity in the Pacific oyster Crassostrea gigas ArchiMer
Ernande, Bruno; Boudry, Pierre; Heurtebise, Serge; Haure, Joel; Martin, Jean-louis.
Introduction : Growth and survival are the life-history traits of the most interest for oyster production since they determine biomass output (growth x survival). This study aimed to investigate whether there is genetic basis for growth, survival and their plasticity to know if a selection program is conceivable for these traits. Material and Methods : Nested half-sibs mating design was used to produce families of oysters: 5 males were each mated to three females producing 5 half-sibs families, each containing 3 full-sib families. At 15 months of age (May 1999), each full-sibs family was randomly split into five groups which were given different treatments. Two groups were placed in a constant environment: one with low food resources and another with high...
Tipo: Text Palavras-chave: Genotyic; Plasticity; Survival; Growth; Genetic; Crassostrea gigas; Pacific oyster.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3498.pdf
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Genetic basis of summer mortality in juvenile cupped oysters ArchiMer
Degremont, Lionel; Boudry, Pierre; Bedier, Edouard; Ropert, Michel; Soletchnik, Patrick; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois.
The French project "Morest", coordinated by IFREMER, aims to understand the causes of the summer mortalities in Crassostrea gigas. In 2001, three sets of families were bred following a nested half-sib mating design. 17 half-sib families (HSF) were obtained in this first generation (G1) and reared in 3 sites. Significant differences in survival were observed among HSF, and some HSF showed high levels of mortality in all sites, clearly indicating a genetic basis for survival. In 2002, a second generation (G2), including divergent selection and inbred lines, was constituted. Monitoring of survival and growth of G2s were the same as in 2001. Significant differences in survival were found between the offspring of the "high" and "low" selected groups and between...
Tipo: Text Palavras-chave: Summer; Oyster culture; Juveniles mortality; Genetics; Commercial species; Breeding.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3346.pdf
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Genetic characterisation of oyster populations along the north-eastern coast of Tunisia ArchiMer
Dridi, S.; Romdhane, M. S.; Heurtebise, Serge; El Cafsi, M.; Boudry, Pierre; Lapegue, Sylvie.
The taxonomy of oysters has been traditionally based on characteristics of the shell. More recently, the analysis of protein and DNA polymorphism has provided a means to overcome difficulties in distinguishing the different species of oysters based solely on shell morphology. In order to identify oysters of the Tunisian north-east coast, we sequenced a 16S rRNA mitochondrial fragment from 68 oysters sampled from the Bizert Lagoon and the Gulf of Hammamet in northern Tunisia. Comparison of oyster 16S rRNA sequences available in GenBank showed the presence of both Ostreola stentina and Crassostrea gigas in our samples, which could not be detected on the basis of shell morphology only. These data confirmed that C. gigas, a non-native species, is now...
Tipo: Text Palavras-chave: 16S rRNA; Crassostrea gigas; Genetics; Mediterranean Sea; Ostreola stentina; Polymorphism.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00000/11063/7820.pdf
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Genetic effect of domestication selective pressures on Pacific oyster at larval stage ArchiMer
Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Boudry, Pierre.
Among bivalve species, the Pacific oyster, Crassostrea gigas, is the most economically important bivalve production over the world. Today, C. gigas is subject to an important production effort that leads to an intensive artificial selection. Larval stage is relatively unknown, specifically in a domestication context. Genetic consequence of artificial selection is still at a preliminary study. We aimed to tackle the consequence of inconscient domestication on the variance reproductive success focusing on larval stage, keystone of the life cycle. We studied two kinds of specific selective processes that common hatchery rearing practices exert : the effect of discarding the smallest larvae on genetic diversity and the artificial environment rearing effect via...
Tipo: Text Palavras-chave: Selection; Genetic correlations; Genetic variability; Genetic; Domestication selective; Crassostrea gigas; Pacific oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3441.pdf
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Genetic impact of the reproduction dynamics in the European flat oyster Ostrea edulis ArchiMer
Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre.
The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to persue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the variance of individual...
Tipo: Text Palavras-chave: Recruitment; Microsatellite markers; Ostrea edulis; European flat oyster; Genetic.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3485.pdf
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Genetic improvement and selection in shellfish : a review based on oyster research and production ArchiMer
Boudry, Pierre; Barre, Marc; Gerard, Andre.
Despite the economic importance of shellfish, genetic improvement has not yet had a great impact on these species. Shellfish farming is traditionally based on wild species whose natural populations are often overexploited and do not fulfill market demand. Up to now, the most effective answer to disease problems or for the improvement of productivity has been the introduction of new species. However, the introduction of new species is constrained by their ecological impact and is also limited by the availability of suitable species. Genetic improvement of local species should therefore be of great importance as a viable alternative for the long term sustainability of the shellfish industry. The different approaches to genetic improvement include...
Tipo: Text Palavras-chave: Selective breeding; Hybridization; Polyplidy; Introduction; Oysters; Selection; Hybridation; Polyploidie; Introduction; Huitres.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3499.pdf
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Genetic improvement and selection in shellfish : a review based on oyster research and production ArchiMer
Boudry, Pierre; Barre, Marc; Gerard, Andre.
Despite the economic importance of shellfish, genetic improvement has not yet had a great impact on these species. Shellfish farming is traditionally based on wild species whose natural populations are often overexploited and do not fulfil market demand. Up to now, the most effective answer 10 disease problems or for the improvement of productivity has been the introduction of new species. However, the introduction of new species is constrained by their ecological impact and is also limited by the availability 01 suitable species. Genetic improvement of local species should therefore be of great importance as a viable alternative for the long term sustainability 01 the shellfish Industry. The different approaches 10 genetic improvement include...
Tipo: Text Palavras-chave: Selective breeding; Hybridization; Polyploidy; Introduction; Oyter; Sélection; Hybridation; Polyploïdie; Introduction; Huîtres.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-2740.pdf
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Genetic improvement of hatchery propagated bivalve stocks : prospects and constraints ArchiMer
Boudry, Pierre.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the production of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or other traits, could be obtained in diploids. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. In the U.S.A., Australia and New Zealand, family-based selective breeding programs have recently been initiated to improve growth, disease tolerance and yield. In Europe, where both natural and...
Tipo: Text Palavras-chave: Microsatellite markers; Crassostrea gigas; Selective breeding; Triploid; Pacific oyster; Genetic.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3451.pdf
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